Commercial Diagnostic Disease Lab

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Description

For more on the ELISA technique visit the University of Wisconsin Plant Pathology Lab website.
A commercial lab such as Agdia, www.agdia.com, has a variety of services that Maria must sort through. She may send a plant sample into the lab or request a test kit that she could use at her greenhouse operation. Assuming Maria elects to send in a plant sample, an ornamental screening test would be prescribed: Agdia states that this is a starting point to identify pathogens. Maria would need to follow the instructions for submitting a sample, which typically require overnight shipping charges to ensure that plant tissue remains in good condition.The Enzyme-Linked Immuno Sorbent Assay (ELISA) testing protocol is a technique that was developed in the late 1970’s to detect viruses.

Results

The ornamental screening test conducted on the sample of several symptomatic dicentra leaves that Maria sent came back negative.

Pathogen Result
Alfalfa Mosaic Virus Negative
Arabis Mosaic Virus Negative
Broad Bean Wilt Virus Negative
Chrysanthemum Virus B Negative
Cucumber Mosiac Virus Negative
Impatiens Necrotic Spot Virus Negative
Polyvirus Group Test Negative
Prunus Necrotic Ringspot Virus Negative
Tobacco Mosiac Virus Negative
Tobacco Ringspot Virus Negative
Tobacco Streak Virus Negative
Tomato Aspermy Virus Negative
Tomato Mosaic Virus Negative
Tomato Ringspot Virus Negative
Tomato Spotted Wilt Virus Negative

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